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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

《农业科学与工程前沿(英文)》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE -2020343

摘要:

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornisXanthium sibiricumArtemisia argyiTupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus nigerBotrytis cinerea, Penicillium digitatumP. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

 

关键词: Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis    

ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

《农业科学与工程前沿(英文)》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE-2020343

摘要: The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of , , , and were evaluated against the mycelial growth of the plant pathogenic fungi , , and . All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol ( ), 95% ethanol ( ), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

关键词: Botrytis cinerea     food preservation     plant diseases     plant pathogenic fungi     Tupistra chinensis    

A Novel Light-Responsive Gene BcCfaS Regulates the Photomorphogenesis and Virulence of Botrytis cinerea

Guangjin Li,Zhanquan Zhang,Yong Chen,Tong Chen,Boqiang Li,Shiping Tian,

《工程(英文)》 doi: 10.1016/j.eng.2023.08.015

摘要: Light is a fundamental environmental factor for living organisms on earth—not only as a primary energy source but also as an informational signal. In fungi, light can be used as an indicator for both time and space to control important physiological and morphological responses. Botrytis cinerea (B. cinerea) is a devastating phytopathogenic fungus that exploits light cues to optimize virulence and the balance between conidiation and sclerotia development, thereby improving its dispersal and survival in ecosystems. However, the components and mechanisms underlying these processes remain obscure. Here, we identify a novel light-signaling component in B. cinerea, BcCfaS, which encodes a putative cyclopropane fatty-acyl-phospholipid synthase. BcCfaS is strongly induced by light at the transcriptional level and plays a crucial role in regulating photomorphogenesis. Deletion of BcCfaS results in reduced vegetative growth, altered colony morphology, impaired sclerotial development, and enhanced conidiation in a light-dependent manner. Moreover, the mutant exhibits serious defects in stress response and virulence on the host. Based on a lipidomics analysis, a number of previously unknown fungal lipids and many BcCfaS-regulated lipids are identified in B. cinerea, including several novel phospholipids and fatty acids. Importantly, we find that BcCfaS controls conidiation and sclerotial development by positively regulating methyl jasmonate (MeJA) synthesis to activate the transcription of light-signaling components, revealing for the first time the metabolic base of photomorphogenesis in fungi. Thus, we propose that BcCfaS serves as an integration node for light and lipid metabolism, thereby providing a regulatory mechanism by which fungi adapt their development to a changing light environment. These new findings provide an important target for antifungal design to prevent and control fungal disease.

关键词: Light     Botrytis cinerea     Cyclopropane fatty-acyl-phospholipid synthase     Lipid metabolism     Photomorphogenesis     Virulence    

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

《环境科学与工程前沿(英文)》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1356-4

摘要:

•Bacterial concentrations from eight stages were 104–105copies/m3.

关键词: Size distribution     Airborne bacteria     Biological diversity     Human pathogenic bacteria     Composting plants    

未来装备医工程思维

高金吉

《中国工程科学》 2003年 第5卷 第12期   页码 30-35

摘要: 将医学科学理论和方法用于装备的故障诊断、预测和防治,有可能逐步形成用工程手段来防治故障的专门用于装备的“医学”——“装备医工程”(PME, plant

关键词: 装备医工程     疾病     故障     防治    

OPPORTUNITIES AND APPROACHES FOR MANIPULATING SOIL-PLANT MICROBIOMES FOR EFFECTIVE CROP NITROGEN USE

《农业科学与工程前沿(英文)》 2022年 第9卷 第3期   页码 333-343 doi: 10.15302/J-FASE-2022450

摘要:

● Matching nitrification inhibitors with soil properties and nitrifiers is vital to achieve a higher NUE.

关键词: nitrogen     microbiome     NUE     rhizosphere     phyllosphere     soil food web    

SUSTAINABLE PLANT PEST MANAGEMENT THROUGH OPTIMIZATION AND MINIMIZATION

Plant Production and Protection Division (NSP), Food and Agriculture Organization of the United Nations

《农业科学与工程前沿(英文)》 2022年 第9卷 第1期   页码 161-166 doi: 10.15302/J-FASE-2021426

摘要:

Plant pests and diseases have significant negative impacts on global food security, world trade and rural livelihoods. Climate change exacerbates these impacts in certain parts of the world. Overreliance on pesticides as the primary tool for plant pest management leads to problems such as pesticide resistance and pest resurgence. Environmental and food safety concerns are also associated with overuse of pesticides in crop production. There is clearly a need for a shift in pest management strategies and practices globally. Optimization of structures and functions in crop production agroecosystems through soil conservation practices and cropping diversification can improve pest regulation services provided in the systems. Prioritization of safer alternatives and practices in the IPM pyramid, such as resistant varieties and biopesticides, helps minimize the use of potentially risky agricultural inputs such as synthetic pesticides. Investment is needed to boost the development of innovative green technologies and practices. Production, distribution, use and regulatory capacities need to be strengthened to facilitate large-scale adoption of green technologies and practices. Finally, policy, financial and market instruments should be wielded to provide an enabling environment for the transformation to sustainable plant pest and disease management strategies and practices worldwide.

 

An introduction to the medicinal plant genome project

Shilin Chen, Li Xiang, Xu Guo, Qiushi Li

《医学前沿(英文)》 2011年 第5卷 第2期   页码 178-184 doi: 10.1007/s11684-011-0131-0

摘要: In recent years, genomics has developed rapidly with the application of next-generation sequencing technology. However, very few studies have been carried out on genomics for medicinal plants. This paper introduces the genome research of medicinal plants, including genome sequencing, assembly, annotation, and functional genomics, to set up the foundation for the development of natural medicines and the selection of cultivars with good agricultural traits. This study places the study on traditional Chinese medicine into the frontier field of life science.

关键词: genomics     medicinal plant     sequencing     application    

HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

《农业科学与工程前沿(英文)》 2022年 第9卷 第1期   页码 98-109 doi: 10.15302/J-FASE-2021389

摘要:

About 80% of plant viruses are transmitted by specific insect vectors, especially hemipterans with piercing-sucking mouthparts. Many virus-transmitting insects are also important crop pests that cause considerable losses in crop production. This review summarizes the latest research findings on the interactions between plant viruses and insect vectors and analyzes the key factors affecting insect transmission of plant viruses from the perspectives of insect immunity, insect feeding, and insect symbiotic microorganisms. Additionally, by referring to the latest applications for blocking the transmission of animal viruses, potential control strategies to prevent the transmission of insect-vectored plant viruses using RNAi technology, gene editing technology, and CRISPR/Cas9+ gene-driven technology are discussed.

 

关键词: control strategies / feeding / immunity / insect vector / microorganism / plant virus    

Intelligent diagnosis methods for plant machinery

Huaqing WANG, Peng CHEN, Shuming WANG,

《机械工程前沿(英文)》 2010年 第5卷 第1期   页码 118-124 doi: 10.1007/s11465-009-0084-z

摘要: This paper reports several intelligent diagnostic approaches based on artificial neural network and fuzzy algorithm for plant machinery, such as the diagnosis method using the wavelet transform, rough sets, and fuzzy neural network; the diagnosis method based on the sequential inference and fuzzy neural network; the diagnosis approach by the possibility theory and certainty factor model; and the diagnosis method on the basis of the adaptive filtering technique and fuzzy neural network. These intelligent diagnostic methods have been successfully applied to condition diagnosis in different types of practical plant machinery.

关键词: intelligent diagnosis     neural network     fuzzy algorithm     adaptive filtering     plant machinery    

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

《农业科学与工程前沿(英文)》 2016年 第3卷 第2期   页码 102-112 doi: 10.15302/J-FASE-2016100

摘要: Plants are frequently affected by pathogen infections. To effectively defend against such infections, two major modes of innate immunity have evolved in plants; pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity. Although the molecular components as well as the corresponding pathways involved in these two processes have been identified, many aspects of the molecular mechanisms of the plant immune system remain elusive. Recently, the rapid development of omics techniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogen interactions from a systems perspective and studies on protein–protein interactions (PPIs) between plants and pathogens have been carried out and characterized at the network level. In this review, we introduce experimental and computational identification methods of PPIs, popular PPI network analysis approaches, and existing bioinformatics resources/tools related to PPIs. Then, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks between plants and pathogens. We anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen interactions and will offer some new opportunities for crop protection and improvement.

关键词: plant–pathogen interactions     systems biology     omics     plant immunity     protein–protein interaction     network    

Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project

Oluwole Alfred OLATUNJI

《工程管理前沿(英文)》 2018年 第5卷 第3期   页码 334-346 doi: 10.15302/J-FEM-2018011

摘要:

This paper uses project organizational theories to draw lessons from a historic megaproject, the Ajaokuta Steel Plant (ASP). Archival reports on the ASP were explored to identify the unique attributes of the project; the political wrangling that underplayed its evolution, its economic significance and organizational impacts. Findings suggest the goals of the ASP project were, and still are, unambiguous. Failure occurred as socio-political forces aggravated the project’s complex milestones. Stakeholders were impatient with pre-project investigations. During planning, owners ignored opinions that were contrary to their expectations. While delays lingered, pressures from the global economy weakened the project’s motivation to succeed. These combined to turn the project’s outcomes into a chaotic situation that triggered dire implications. Despite about 1400% overrun in cost, the success achieved on the plant was 28% at commissioning. Contractors remained on site until eight years after commissioning. Six key elements of the 482 items in the ASP project contract were not delivered nearly 40 years on. A simplistic look at these suggests poor planning is the main problem. However, planning issues is not entirely strange in greenfield projects. The paper draws strength from project organization theories to explain what was poor about the planning. Socrates’ generic management theory was used to explain the role of leadership in the failure of the ASP project. McGregor’s Theory X and Theory Y explain the significance of stakeholders’ integration in megaprojects. Systems and chaos theories were used to explain the sensitivity of the ASP project to uncertainties. Narratives on these combine well to inspire stakeholders of megaprojects on where and how to seek courage in making effective plans that can help achieve success in complex projects. While normative literature only recognizes project success in a definitive perspective, this study provides insights from failure as an instrument to trigger sublime reflections.

关键词: industrial projects     megaprojects     Nigeria     project organizational theories     steel plant    

Rhizosphere immunity: targeting the underground for sustainable plant health management

Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN

《农业科学与工程前沿(英文)》 2020年 第7卷 第3期   页码 317-328 doi: 10.15302/J-FASE-2020346

摘要:

Managing plant health is a great challenge for modern food production and is further complicated by the lack of common ground between the many disciplines involved in disease control. Here we present the concept of rhizosphere immunity, in which plant health is considered as an ecosystem level property emerging from networks of interactions between plants, microbiota and the surrounding soil matrix. These interactions can potentially extend the innate plant immune system to a point where the rhizosphere immunity can fulfil all four core functions of a full immune system: pathogen prevention, recognition, response and homeostasis. We suggest that considering plant health from a meta-organism perspective will help in developing multidisciplinary pathogen management strategies that focus on steering the whole plant-microbe-soil networks instead of individual components. This might be achieved by bringing together the latest discoveries in phytopathology, microbiome research, soil science and agronomy to pave the way toward more sustainable and productive agriculture.

关键词: rhizosphere     soil microbiome     plant immunity     microbial ecology     plant health     soilborne pathogens    

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

《农业科学与工程前沿(英文)》 2022年 第9卷 第1期 doi: 10.15302/J-FASE- 2021390

摘要:

It has previously been shown that tryptophan, the biosynthesis precursor of Peganum harmala alkaloids, and its derivatives have anti-TMV activity both in vitro and in vivo. Further exploration of this led to the identification of NK0238 as a highly effective agent for the prevention and control of diseases caused by plant viruses, but the existing routes are unsuitable for its large-scale synthesis. This study optimized a route for two-step synthesis of this virucide candidate via reaction of l-tryptophan with triphosgene to produce l-tryptophan-N-carboxylic anhydride, which then reacts with n-octylamine to give NK0238 at up to 94% yield and nearly 97% HPLC purity. In addition, the route was used for the preparation of NK0238 on a>40 g scale permitting further assessment of its antivirus activity in the greenhouse and field experiments, and toxicity tests. NK0238 exhibited useful antiviral activities against a variety of viruses both in greenhouse and field experiments. The toxicity tests showed that NK0238 was not acutely toxic to birds, fish, honey bees and silkworms. The optimized route provides a solid foundation for its large-scale synthesis and subsequent efficacy and toxicity studies, its excellent activity and safety make NK0238 a promising drug candidate for further development.

 

关键词: antiviral plant protectant / antiviral in the greenhouse / field evaluation / l-trp-NCA / synthesis optimization / toxicity tests    

Decontamination efficiency and root structure change in the plant-intercropping model in vertical-flow

Yonghua CHEN, Xiaofu WU, Mingli CHEN, Kelin LI, Jing PENG, Peng ZHAN

《环境科学与工程前沿(英文)》 2013年 第7卷 第6期   页码 906-912 doi: 10.1007/s11783-013-0579-z

摘要: Subtropical climatic conditions can contribute to the death of the aerial parts of constructed wetland plants in winter. This presents a barrier to the widespread application of constructed wetland and is an issue that urgently needs to be solved. Three contrasting experiments, the plant-intercropping model (A), the warm-seasonal plant model (B), and the non-plant model (C), were studied in terms of their efficiency in removing pollutants, and the change in root structure of plants in the plant-intercropping model within the vertical-flow constructed wetlands. The results indicate that model A was able to solve the aforementioned problem. Overall, average removal rates of three pollutants (COD , total nitrogen (TN) and total phosphorous (TP)) using model A were significantly higher than those obtained using models B and C ( <0.01). Moreover, no significant differences in removal rates of the three pollutants were detected between A and B during the higher temperature part of the year ( >0.05). Conversely, removal rates of the three pollutants were found to be significantly higher using model A than those observed using model B during the lower temperature part of the year ( <0.01). Furthermore, the morphologies and internal structures of plant roots further demonstrate that numerous white roots, whose distribution in soil was generally shallow, extend further under model A. The roots of these aquatic plants have an aerenchyma structure composed of parenchyma cells, therefore, roots of the cold-seasonal plants with major growth advantages used in A were capable of creating a more favorable vertical-flow constructed wetlands media-microenvironment. In conclusion, the plant-intercropping model (A) is more suitable for use in the cold environment experienced by constructed wetland during winter.

关键词: vertical-flow constructed wetlands     plant intercropping model     warm seasonal plant model    

标题 作者 时间 类型 操作

IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

期刊论文

ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

期刊论文

A Novel Light-Responsive Gene BcCfaS Regulates the Photomorphogenesis and Virulence of Botrytis cinerea

Guangjin Li,Zhanquan Zhang,Yong Chen,Tong Chen,Boqiang Li,Shiping Tian,

期刊论文

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

期刊论文

未来装备医工程思维

高金吉

期刊论文

OPPORTUNITIES AND APPROACHES FOR MANIPULATING SOIL-PLANT MICROBIOMES FOR EFFECTIVE CROP NITROGEN USE

期刊论文

SUSTAINABLE PLANT PEST MANAGEMENT THROUGH OPTIMIZATION AND MINIMIZATION

Plant Production and Protection Division (NSP), Food and Agriculture Organization of the United Nations

期刊论文

An introduction to the medicinal plant genome project

Shilin Chen, Li Xiang, Xu Guo, Qiushi Li

期刊论文

HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

期刊论文

Intelligent diagnosis methods for plant machinery

Huaqing WANG, Peng CHEN, Shuming WANG,

期刊论文

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

期刊论文

Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project

Oluwole Alfred OLATUNJI

期刊论文

Rhizosphere immunity: targeting the underground for sustainable plant health management

Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN

期刊论文

ROUTE DEVELOPMENT, ANTIVIRAL STUDIES, FIELD EVALUATION AND TOXICITY OF AN ANTIVIRAL PLANT PROTECTANT

期刊论文

Decontamination efficiency and root structure change in the plant-intercropping model in vertical-flow

Yonghua CHEN, Xiaofu WU, Mingli CHEN, Kelin LI, Jing PENG, Peng ZHAN

期刊论文